FIGURE

Fig. 1

ID

ZDB-FIG-170420-8

Publication

Zhu et al., 2017 - Vegfa Impacts Early Myocardium Development in Zebrafish

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Fig. 1

Vascular endothelial growth factor Aa (vegfaa) deficiency disrupts myocardial and endocardial morphogenesis. (A–I) Fluorescence micrographs of the hearts of wild-type (A–C), vegfaa mutant (D–F), and vegfaa morphants (G–I) at 96 hpf (hours post-fertilization) with the background of Tg(myl7:mCherry)/Tg(kdrl:EGFP) transgenic fish. The whole heart is substantially small and linearized in the mutant and the morphant, and no cavity is visible inside the heart lumen. Scale bar: 50 μm (A– I); (J,K) In situ hybridization of vegfaa in wild-type embryos at 18-somite stage. vegfaa is expressed in anterior lateral plate mesoderm as indicated by the arrow. (J) Lateral views, (K) Dorsal views with the anterior at the top. Scale bar: 200 μm (J,K). WT, wild-type; mo, morpholino

Expression Data

Genes:	GFP mCherry vegfaa
Fish:	AB la116Tg; sd7Tg la116Tg; sd7Tg + MO9-vegfaa vegfaa^{zf1090/zf1090}; la116Tg; sd7Tg
Knockdown Reagent:	MO9-vegfaa
Anatomical Terms:	anterior lateral plate mesoderm endocardium myocardium
Stage Range:	14-19 somites to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la116Tg; sd7Tg + MO9-vegfaa vegfaa^{zf1090/zf1090}; la116Tg; sd7Tg
Knockdown Reagent:	MO9-vegfaa
Observed In:	atrium cardiac ventricle endocardium endothelial cell heart heart anatomical space heart tube formation heart looping heart valve
Stage:	Day 4

Phenotype Detail

Acknowledgments

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