FIGURE

Fig. 6

ID

ZDB-FIG-170220-8

Publication

Mayrhofer et al., 2017 - A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK/PI3K induced malignant growth

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Fig. 6

YAP activation promotes tumour growth. (A) Schematic representation of tumour induction through the co-injection of oncogenic constructs (green) into zic:Gal4 embryos. (B) Quantification of BrdU-positive cells in the telencephalon of 3 and 14 dpf juveniles reveals doubling in the number of proliferating cells in zic:RAS,YAP_somatic fish compared with zic:RAS_somatic fish and zic:Gal4 control fish. (C) Confocal image of 14 dpf fish showing BrdU expression (magenta) in zic:Gal4, zic:RAS_somatic and zic:RAS,YAP_somatic. (D) Survival rate of zic:RAS_somatic (green dashed line; n=166) compared with zic:Gal4 (black solid line, black asterisk; n=105) and zic:RAS,YAP_somatic (red dashed line, red asterisks; n=100). (E) Graph representing the frequency of different lesions resulting from zic:RAS,YAP_somatic expression as determined by stereomicroscopic analysis with tumours in telencephalon (T), diencephalon (D) and IVth ventricle (V), and heterotopia (H). (F) Immunostaining for GFP, BrdU, GFAP and HU-C of zic:RAS,YAP_somatic fish. Data are represented as mean±s.d. *P<0.05; ***P<0.001. Scale bars: 20 µm in C,F.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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