Fig. 1

ljr^p23ahub mutants display an increased number and uniform distribution of rod photoreceptors.

(A) Confocal immunofluorescent images labeled for rods (red) and UV-sensitive cones (green) from WT, ljr^p23ahub, lor^p25bbtl and homozygous lor^p25bbtl/ljr^p23ahub retinas at 4 days-post-fertilization (dpf). WT larvae show asymmetric rod distribution in central and dorsal retina and a uniform distribution of UV-sensitive cones across the entire retina. ljr^p23ahub and lor^p25bbtl mutants demonstrate an increased number and uniform arrangement of rods but fewer UV-sensitive cones labeled in lor^p25bbtl. Double-mutant retinas display higher rod labeling and few UV-sensitive cones. (B) Graph showing the average number of rods per unit area dorsal to the optic nerve (WT, n = 5; ljr^p23ahub, n = 5; lor^p25bbtl, n = 5; ljr^p23ahub/lor^p25bbtl, n = 5). Significantly-different means by one-way ANOVA with Tukey’s post-hoc test, b vs c, p<0.05, all other comparisons p<0.0001. (C) Graph showing the average number of UV-sensitive cones per unit area from samples used in 1B, a vs b, p<0.0001. (D) Graph showing the average number of rods per unit area (WT, n = 5; ljr^p23ahub/+, n = 6; ljr^p23ahub, n = 5); a vs b, p<0.05, all other comparisons p<0.0001. (E) Flat mount views of confocal immunofluorescent images labeled for red-sensitive (brighter signal) and green -sensitive (dimmer signal) cones from WT and ljr^p23ahub retinas at 4 dpf. ljr^p23ahub mutants maintain the alternating arrangement of red- and green-sensitive cones. (F) Graph showing the average number of red- and green-sensitive cones per unit area (WT, n = 6; ljr^p23ahub, n = 6). No significant differences are observed, Student’s t test, p>0.05. Error bars represent SD.