FIGURE

Fig. S2

ID

ZDB-FIG-170208-7

Publication

Dona et al., 2015 - NINL and DZANK1 Co-function in Vesicle Transport and Are Essential for Photoreceptor Development in Zebrafish

Other Figures

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Fig. S2

Recapitulation of the phenotype by a second dzank1 spMO targeting exon4.

Injection of a dzank1 ex4 spMO-injected larvae completely recapitulates the phenotype observed in dzank1 ex8 spMO-injected larvae, including pericardial edema, small eyes, defects in ambulatory activity (b) and shortened photoreceptor outer segments (b') as compared to control MO-injected larvae from the same clutch (a,a'). (c) Characterization of the effect of the dzank1 ex4 spMO at 2 dpf by non-quantitative RT-PCR analysis. Injection of various amounts of MO resulted in the (partial) skipping of exon4, leading to a premature termination of translation. PCR fragments were analyzed by Sanger sequencing. Scale bars represent 500 μm (a-b) and 15 μm (a'-b').

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL + MO1-dzank1
Knockdown Reagent:	MO1-dzank1
Observed In:	eye eye photoreceptor cell photoreceptor outer segment pericardium swimming
Stage:	Day 4

Phenotype Detail

Acknowledgments

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