ZFIN Image: Dona et al., 2015, Fig. S2

Image

Figure Caption

Fig. S2

Recapitulation of the phenotype by a second dzank1 spMO targeting exon4.

Injection of a dzank1 ex4 spMO-injected larvae completely recapitulates the phenotype observed in dzank1 ex8 spMO-injected larvae, including pericardial edema, small eyes, defects in ambulatory activity (b) and shortened photoreceptor outer segments (b') as compared to control MO-injected larvae from the same clutch (a,a'). (c) Characterization of the effect of the dzank1 ex4 spMO at 2 dpf by non-quantitative RT-PCR analysis. Injection of various amounts of MO resulted in the (partial) skipping of exon4, leading to a premature termination of translation. PCR fragments were analyzed by Sanger sequencing. Scale bars represent 500 μm (a-b) and 15 μm (a'-b').

Figure Data

Phenotype details

Acknowledgments

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