Fig. 9

Inhibition of RhoA/ROCK pathway affects remodeling of the furrow microtubule array. Buffer (A, C and E) and C3, Rhosin and h1152 (B, D and F respectively) injected embryos labeled with anti α tubulin antibody. In buffer injected embryos the furrow microtubule array form as a parallel array at the center of the cleavage furrow at mature furrow (A, a1; C, c1 and E, e1) meanwhile in injected embryos with C3 (B, b1), Rhosin (D, d1) or h1152 (F, f1) no deposition of the microtubules at the center of the cleavage furrow was observed. Upon furrow maturation furrow microtubules appear at the distal edge in a characteristic V shape in buffer injected embryos (A, a2; C, c2 and E, e2). Microtubules from injected embryos with C3, Rhosin or h1152 did not showed this characteristic pattern (B, b2; D, d2 and F, f2 respectively) but maintained a perpendicular arrangement towards the cleavage furrow (b2 and d2) or showed a complete lack of organization (f2). Data was analyzed from 3 independent experiments of C3 injected embryos (n=11) or buffer injected embryos as control (n=7); from 4 independent experiments of Rhosin injected embryos (n=12) or buffer injected embryos as control (n=15) and from 4 independent experiments of h1152 injected embryos (n=13) or buffer injected as control (n=11).