Fig. 6
- ID
- ZDB-FIG-170123-14
- Publication
- Miranda-Rodríguez et al., 2017 - RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos
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Rhosin injections at 1-cell stage cause significant decrease of primordial germ cells (PGCs) in 24-h zebrafish embryos. (A and E) nanos mRNA in situ hybridization in 24-hpf zebrafish embryos showed the normal staining of the PGCs in embryos at the prospective gonads. Injected embryos with Rhosin showed less nanos staining at only one side of the embryo and signal was not detected at the other side (B and F) Decrease in PGCs is evident in Rhosin treated embryos as detected by nanos mRNA in situ hybridization in embryos (C and G). Ectopic signal of nanos is shown with arrowhead (C and G). Embryos with out positive nanos cells (D and H). (A-D) lateral view and (E-H) dorsal views. (I) The embryos with normal nanos localization of injected control embryos (n=79) and Rhosin injected embryos (n=83) were quantified in 5 independent experiments. The error bars represent the standard error of the mean (** p<0.01). |
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| Stage: | Prim-5 |
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| Stage: | Prim-5 |
Reprinted from Developmental Biology, 421(1), Miranda-Rodríguez, J.R., Salas-Vidal, E., Lomelí, H., Zurita, M., Schnabel, D., RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos, 27-42, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.