FIGURE

Fig. 8

ID

ZDB-FIG-170123-16

Publication

Miranda-Rodríguez et al., 2017 - RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos

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Fig. 8

Inhibition of ROCK activity generates embryos with less vasa positive cells. Characteristic vasa staining at 24 hpf embryos showing localization at the beginning of the yolk extension in lateral view (A and a) and at the dorsal view two groups of cells mark the prospective gonads at each side of embryo (C and c). Injected embryos with h1152 showed less vasa positive cells (B and b), and at the dorsal view injected embryo with h1152 showed vasa positive cells only at one of the prospective gonads (D and d). Also ectopic signal was observed in injected embryos with h1152 (E). Embryos with the normal vasa localization at 24 hpf were quantified in injected embryos with control buffer (n=85) or in embryos injected with h1152 (n=93) and statistical difference were observed in 3 independent experiments. The error bars represent the standard error of the mean (** p<0.01).

Expression Data

Gene:	nanos3
Fish:	WT
Condition:	chemical treatment by injection: (S)-2-methyl-1-(4-methylisoquinoline-5-sulfonyl)-1,4-diazepane dihydrochloride
Anatomical Term:	primordial germ cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by injection: (S)-2-methyl-1-(4-methylisoquinoline-5-sulfonyl)-1,4-diazepane dihydrochloride
Observed In:	primordial germ cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 421(1), Miranda-Rodríguez, J.R., Salas-Vidal, E., Lomelí, H., Zurita, M., Schnabel, D., RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos, 27-42, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.