FIGURE

Fig. S5

ID

ZDB-FIG-170104-19

Publication

Jim�nez-Amilburu et al., 2016 - In�Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

Notch inhibition does not affect cardiomyocyte apical constriction

(A-C') Confocal images (mid-sagittal sections) of 81 hpf Tg(myl7:MKATE-CAAX);Tg(TP1:VenusPEST) zebrafish hearts. (A-A') DMSO-treated larvae showing TP1:VenusPEST positive cardiomyocytes (A and A', asterisks), endocardial cells (A and A', white arrows) and AV valve cells (A and A', yellow arrows). (B- C'') Embryos were treated starting at 48 hpf with 50 ?M DAPT (B-B') or 10 ?M LY411575 (C-C') and imaged at 81 hpf. In both sets of experimental animals, Notch activation was not observed in cardiomyocytes (B' and C', asterisks). (D) Number of apical constrictions after 50 ?M DAPT or 10 ?M LY411575 inhibitor treatments at 79 hpf. Each dot represents one heart. Data are shown as mean � SEM. ns, no significant differences by Student's t-test. V, ventricle; AVC, atrioventricular canal. Scale bars, 20 ?m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.