FIGURE

Fig. 2

ID

ZDB-FIG-161122-38

Publication

Mouti et al., 2016 - Minimal contribution of ERK1/2-MAPK signalling towards the maintenance of oncogenic GNAQQ209P-driven uveal melanomas in zebrafish

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Fig. 2

Oncogenic GNAQ^Q209P-mediated activation of ERK and YAP signalling in choroidal melanocytes at the junction between RPE and choroid. A-D. Transverse sections of formalin-fixed and paraffin-embedded eye tissues of F₁ generation, 5-month-old Tg (mitfa:GNAQ^Q209P) zebrafish. (A) H&E staining demonstrating choroidal hyperplasia (black arrowheads). White dashed box indicates the region of the choroid magnified in B-D. (B-D) Transverse sections of formalin-fixed and paraffin-embedded eye tissues were stained by IHC, visualized by ImmPact NovaRed peroxidase (HRP) substrate then counterstained with hematoxylin (blue). (B) Negative control: section incubated with 1x PBS instead of primary antibody. (C) Immunoreactivity to pERK1/2 (read-out of ERK activation; black arrowheads) in melanocytes at the interface between the RPE and choroid. (D) YAP-positive nuclei (read-out of YAP activation; black arrowheads) in the same cells. Scale bars, 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	umc9Tg
Observed In:	eye ERK1 and ERK2 cascade eye hippo signaling melanocyte ERK1 and ERK2 cascade melanocyte hippo signaling
Stage:	Adult

Phenotype Detail

Acknowledgments

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