Fig. 6

sox9b haploinsufficiency impedes the effects of RA on CAC differentiation. (A) Immunofluorescence staining using a Sox9b antibody in 5 dpf pancreata expressing Tp1:nuc-mCherry. Sox9b expression co-localizes with nuclear mCherry in CACs (white arrows). Pancreas is outlined in dashed line. Scale bar=20 µm. (B) Western blot demonstrating that Sox9b expression is reduced in sox9b^fh313 heterozygous larvae. (C-F) Images of dissected pancreata from 5 dpf larvae with the following genotype: (C-D) wildtype (sox9b^+/+) (E-F) sox9b^fh313 heterozygous (sox9b^+/). in situ hybridization for neuroD was used to detect 2° islets. Larvae of differing genotypes (as indicated) were treated from 3 to 5 dpf with either DAPT (C, E) alone or RA+DAPT (D, F). 2° islets are indicated by black arrow heads. White dashes outline the pancreata. Scale bar=100 µm. (G) Numbers of CACs quantified using expression of Tp1:eGFP in pancreata from 5 dpf larvae, genotyped as indicated. Heterozygotes do not have significantly different numbers of CACs compared to wildtype. (H) Numbers of 2° islets/pancreas from genotyped larvae after being treated with DAPT alone or RA+DAPT. Error bar represents SD; **p<0.001.