FIGURE

Fig. 4

ID

ZDB-FIG-161027-4

Publication

Huang et al., 2016 - Sox9b is a mediator of retinoic acid signaling restricting endocrine progenitor differentiation

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Fig. 4

Inducible expression of Sox9b and truncated Sox9b in CAC pancreatic progenitors. (A, B and D, E) Confocal images of 5 dpf pancreata dissected from double transgenic larvae for the Notch-responsive creER^T2driver and either sox9b cre-responder (A, B) or trsox9b cre-responder (D, E) showing localization of Nkx6.1 (green) and mCherry (red). Transgenic larvae were treated with vehicle (A, D) or 4OHT (B, E) from 2 to 3 dpf. The numbers of double positive (green/red) cells were quantified (C, F). Overexpression of Sox9b did not significantly affect numbers of Nkx6.1 positive CACs (C); however, 4OHT induction of trSox9b significantly reduced CAC numbers, an effect that was heightened if 4OHT treatment was performed even earlier in development (1.5-2.5 dpf, F). Scale Bar=100 µm, *p<0.05, **p<0.001.

Expression Data

Gene:	mCherry
Antibody:	Ab2-nkx6
Fish:	jh12Tg; jh47Tg jh12Tg; jh48Tg
Condition:	chemical treatment by environment: afimoxifene
Anatomical Term:	pancreatic centroacinar cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh12Tg; jh48Tg
Condition:	chemical treatment by environment: afimoxifene
Observed In:	pancreatic centroacinar cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 418(1), Huang, W., Beer, R.L., Delaspre, F., Wang, G., Edelman, H.E., Park, H., Azuma, M., Parsons, M.J., Sox9b is a mediator of retinoic acid signaling restricting endocrine progenitor differentiation, 28-39, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.