FIGURE

Fig. S2

ID

ZDB-FIG-161018-6

Publication

Lu et al., 2016 - IGFBP1 increases β-cell regeneration by promoting α- to β-cell transdifferentiation

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Fig. S2

The IGF1R inhibitor PPP increases βcell regeneration and coexpression of insulin and pdx1 with glucagon

A-C. Pdx1 expression in 6dpf larvae during regeneration. Tg(ins:dsRed);Tg(gcg:GFP);Tg(ins:FlagNTR) larvae were treated with MTZ from 3 to 4 dpf to ablate the ² cells and subsequently treated with DMSO or the IGF1R inhibitor PPP from 4 to 6 dpf. Representative confocal images (A, B) at 6 dpf of DMSO or PPPtreated larvae. A gcg⁺ins⁺pdx1⁺ cell is indicated by an arrow. Scale bars: 15 µm. (C) Quantification of gcg⁺, gcg⁺ins⁺, and pdx1⁺gcg⁺ cells (P = 0.8409, *P = 0.0232, and ***P = 0.0007, respectively) in DMSO and PPPtreated groups. n = 20 for each group. Results are presented as mean values ± SEM and analyzed with ANOVA. Related to Fig 4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

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