ZFIN Image: Lu et al., 2016, Fig. S2

Image

Figure Caption

Fig. S2

The IGF1R inhibitor PPP increases βcell regeneration and coexpression of insulin and pdx1 with glucagon

A-C. Pdx1 expression in 6dpf larvae during regeneration. Tg(ins:dsRed);Tg(gcg:GFP);Tg(ins:FlagNTR) larvae were treated with MTZ from 3 to 4 dpf to ablate the ² cells and subsequently treated with DMSO or the IGF1R inhibitor PPP from 4 to 6 dpf. Representative confocal images (A, B) at 6 dpf of DMSO or PPPtreated larvae. A gcg⁺ins⁺pdx1⁺ cell is indicated by an arrow. Scale bars: 15 µm. (C) Quantification of gcg⁺, gcg⁺ins⁺, and pdx1⁺gcg⁺ cells (P = 0.8409, *P = 0.0232, and ***P = 0.0007, respectively) in DMSO and PPPtreated groups. n = 20 for each group. Results are presented as mean values ± SEM and analyzed with ANOVA. Related to Fig 4.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO J.