FIGURE

Fig. 7

ID

ZDB-FIG-160909-7

Publication

Noonan et al., 2016 - Loss of vhl in the zebrafish pronephros recapitulates early stages of human clear cell renal cell carcinoma

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Fig. 7

Proximal vhl^-/- pronephric tubules exhibit improved phenotype when treated with HIF2a inhibitor Compound 76. (A) Treatment protocol. Vhl^-/- embryos were treated with 1µM Compound 76 (76) or DMSO vehicle control from 6hpf to 5.5dpf. (B) H&E staining of 5.5dpf vhl^-/- proximal pronephric tubules (red boxes) treated with Compound 76 compared to vhl^-/- treated with DMSO vehicle control. Scale bars: 0.05mm. (C) Quantification of mean lumen diameter length (transverse section) in 5.5dpf vhl^-/- larvae treated with Compound 76 or DMSO control. (D) Quantification of mean lumen diameter width (transverse section) in 5.5dpf vhl^-/- treated with Compound 76 or DMSO control. Each experiment had at least five larvae per sample group and was performed in duplicate. Data represent mean±s.e.m. *P<0.05, **P<0.01 (paired two-tailed t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vhl^{hu2117/hu2117}
Condition:	chemical treatment by environment: EC 1.14.11.29 (hypoxia-inducible factor-proline dioxygenase) inhibitor
Observed In:	pronephric proximal convoluted tubule pronephric proximal convoluted tubule ciliated epithelial cell pronephric proximal convoluted tubule cytoplasmic vesicle pronephric proximal convoluted tubule epithelial cell pronephric proximal straight tubule pronephric proximal straight tubule ciliated epithelial cell pronephric proximal straight tubule cytoplasmic vesicle pronephric proximal straight tubule epithelial cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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