FIGURE

Fig. 6

ID

ZDB-FIG-160909-6

Publication

Noonan et al., 2016 - Loss of vhl in the zebrafish pronephros recapitulates early stages of human clear cell renal cell carcinoma

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Fig. 6

Proximal vhl^-/- pronephric tubules have increased cell death at 3.5 and 5.5dpf compared to wt siblings. (A-F) Caspase 3 immunofluorescence was performed in whole-mount 3.5, 5.5 and 8.5dpf Tg(ATPase1.a1A4:GFP) vhl^-/- and wt siblings. Caspase 3 staining intensity (red) was then quantified within an ROI (yellow box) encompassing the proximal pronephros (green) using ImageJ. Merged caspase 3 (red) and GFP (green) staining in 5.5dpf wt sibling (A) and vhl^-/- (D) larvae. Caspase 3 staining only is shown in 5.5dpf wt sibling (B) and vhl^-/- (E). GFP staining only is shown in 5.5dpf wt sibling (C) and vhl^-/- (F). (G) Quantification of the mean caspase 3 staining intensity within a ROI in 3.5dpf vhl^-/- versus wt siblings, (H) 5.5dpf vhl^-/- versus wt siblings and (I) 8.5dpf vhl^-/- versus wt siblings. To assist with visualization of caspase 3 staining, the contrast of this image has been adjusted globally using ImageJ software. The quantitative analysis presented was performed on non-enhanced images. Each 3.5, 5.5 and 8.5dpf experiment had ten larvae per sample group and was performed in duplicate. Data represent mean±s.e.m. *P<0.05 (paired two-tailed t-test).

Expression Data

Gene:	EGFP
Antibody:	Ab1-casp3
Fish:	vhl^{hu2117/hu2117} vhl^{hu2117/hu2117}; zf34Tg
Anatomical Term:	pronephric tubule
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vhl^{hu2117/hu2117}; zf34Tg
Observed In:	pronephric proximal convoluted tubule apoptotic process pronephric proximal straight tubule apoptotic process
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.