FIGURE

Fig. 1

ID

ZDB-FIG-160909-1

Publication

Noonan et al., 2016 - Loss of vhl in the zebrafish pronephros recapitulates early stages of human clear cell renal cell carcinoma

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Fig. 1

Proximal vhl^-/- pronephric tubules have distorted architecture and contain cytoplasmic vesicles when compared to wt siblings. (A) Location of the proximal and distal pronephric tubules in the zebrafish larval pronephric kidney. Kidney segments are color coded. The glomerulus (G, black), neck (N, orange), proximal convoluted tubule (PCT, red), proximal straight tubule (PST, blue), distal early (DE, green), distal late (DL, yellow), collecting duct (CD, purple) are highlighted. (B,C) 5.5dpf vhl^-/- and wt siblings were fixed and embedded in JB-4 and sectioned with a glass knife. H&E staining was performed and measurements were taken using ImageJ. H&E staining of wt sibling (B) and vhl^-/- (C) proximal pronephric tubules (red boxes). Scale bars: 0.05mm. (D) Diameter (mm) of proximal pronephric tubule in the transverse section. (E) Mean number of cells per transverse section. Each experiment had at least three larvae per sample group and was performed in duplicate. Data represent mean±s.e.m. *P<0.05, **P<0.01 (paired two-tailed t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vhl^{hu2117/hu2117}
Observed In:	pronephric proximal convoluted tubule pronephric proximal convoluted tubule cytoplasmic vesicle pronephric proximal convoluted tubule epithelial cell pronephric proximal straight tubule pronephric proximal straight tubule cytoplasmic vesicle pronephric proximal straight tubule epithelial cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.