FIGURE

Fig. 2

ID

ZDB-FIG-160826-2

Publication

Talbot et al., 2016 - Pharyngeal morphogenesis requires fras1-itga8- dependent epithelial-mesenchymal interaction

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Fig. 2

Comparison of skeletal, endodermal, and tail ectoderm phenotypes between wild type, itga8^b1161 mutants, fras1^b1048 mutants, and itga8^b1161;fras1^b1048 double mutants. (A) Illustration of a zebrafish larva, indicating regions shown in subsequent (B-I) panels. (B-E) Skeletal morphology is revealed using sox9a:EGFP expression (cartilage) and alizarin red staining (bone) at 7 dpf; itga8 and fras1 single and double mutants display similar cartilage defects, in particular, Meckel’s-palatoquadrate joint fusion (arrowhead) and symplectic-ceratohyal fusions (asterisk). (F-I) Bright field images showing normal fin fold morphology (outlined blue) in (F) wild type and (G) itga8^b1161 individuals versus the “blister phenotypes” in (H) fras1^b1048 and (I) itga8^b1161;fras1^b1048 mutants. Scale bars (E and I) are 100 µm. Scale bar in E applies to B-E; Scale bar in I applies to F-I.

Expression Data

Gene:	GFP
Fish:	sox9a^zc81Tg (AB) fras1^b1048/b1048; sox9a^zc81Tg (AB) itga8^b1161/b1161; sox9a^zc81Tg (AB) fras1^b1048/b1048; itga8^b1161/b1161; sox9a^zc81Tg (AB)
Anatomical Term:	pharyngeal arch cartilage
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fras1^b1048/b1048; sox9a^zc81Tg (AB) itga8^b1161/b1161; sox9a^zc81Tg (AB) fras1^b1048/b1048; itga8^b1161/b1161; sox9a^zc81Tg (AB)
Observed In:	caudal fin ceratohyal cartilage hyosymplectic cartilage Meckel's cartilage palatoquadrate cartilage
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 416(1), Talbot, J.C., Nichols, J.T., Yan, Y.L., Leonard, I.F., BreMiller, R.A., Amacher, S.L., Postlethwait, J.H., Kimmel, C.B., Pharyngeal morphogenesis requires fras1-itga8- dependent epithelial-mesenchymal interaction, 136-48, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.