FIGURE

Fig. 7

ID

ZDB-FIG-160817-48

Publication

Naylor et al., 2016 - BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis

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Fig. 7

RA acts downstream of the BMP signalling pathway during kidney patterning.

(a) Whole-mount double in situ hybridization analysis for nephron segment markers slc4a4 (red) and slc12a3 (purple) in embryos at the 15-somites stage injected with bmp2b mRNA or treated with DM dihydrochloride, DEAB and DMSO (vehicle control) at the indicated stages. Embryos are shown as dorsal views with anterior to the left. (b) Embryos were treated as shown and stopped at the 15-somites stage for double in situ hybridization staining for the anterior pronephros marker slc4a4 and the distal pronephros marker slc12a3. All panels are dorsal views of flatmounted embryos. Scale bar, 200 µm.

Expression Data

Genes:	slc4a4a slc12a3
Fish:	TU
Conditions:	chemical treatment by environment: 4-(diethylamino)benzaldehyde chemical treatment by environment: retinoic acid chemical treatment by environment: dorsomorphin chemical treatment by environment: dorsomorphin
Anatomical Terms:	pronephric distal late tubule pronephric proximal convoluted tubule pronephric proximal straight tubule
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU
Conditions:	chemical treatment by environment: 4-(diethylamino)benzaldehyde chemical treatment by environment: retinoic acid chemical treatment by environment: dorsomorphin chemical treatment by environment: dorsomorphin
Observed In:	pronephric distal late tubule pronephric proximal convoluted tubule pronephric proximal straight tubule
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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