Fig. 3
- ID
- ZDB-FIG-160817-44
- Publication
- Naylor et al., 2016 - BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis
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The zulu marker is highly expressed in the PLM. (a) Whole-mount stage series in situ hybridization detecting gene expression of zulu suggest the zuluhigh PLM region at 85% epiboly forms the zuluhigh PLM at somitogenesis stages. All embryos are flat-mounted, 85% epiboly embryos are orientated with animal pole on top and vegetal pole at the bottom, later stages are dorsal views with anterior to the left. (b) Whole-mount double in situ hybridization at the 10-somites stage to detect transcripts for zulu and pax2a/gata1/nkx2.5/tbx5a. All embryos are flat-mounted and shown in dorsal views with anterior to the left. Scale bars, 200 µm. |
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Stage Range: | 75%-epiboly to 10-13 somites |