Fig. 3
The zulu marker is highly expressed in the PLM.
(a) Whole-mount stage series in situ hybridization detecting gene expression of zulu suggest the zuluhigh PLM region at 85% epiboly forms the zuluhigh PLM at somitogenesis stages. All embryos are flat-mounted, 85% epiboly embryos are orientated with animal pole on top and vegetal pole at the bottom, later stages are dorsal views with anterior to the left. (b) Whole-mount double in situ hybridization at the 10-somites stage to detect transcripts for zulu and pax2a/gata1/nkx2.5/tbx5a. All embryos are flat-mounted and shown in dorsal views with anterior to the left. Scale bars, 200 µm.