FIGURE

Fig. 5

ID

ZDB-FIG-160503-8

Publication

Valdivia et al., 2016 - Antagonism between Gdf6a and retinoic acid pathways controls timing of retinal neurogenesis and growth of the eye in zebrafish

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Fig. 5

Pharmacological inhibition of the RA pathway partially rescues the gdf6a mutant CMZ phenotype. (A-F) Lateral views of eyes from 60hpf gdf6a^u768 mutants treated with vehicle (DMSO;A,C,E) or the RAR inverse agonist BMS493 (15µM; B,D,F) showing expression of col15a1b (A,B), ccnd1 (C,D) and atoh7 (E,F). The number of embryos with staining pattern similar to that shown is indicated as a fraction in lower right corner of images. (G) qPCR quantification of relative gene expression levels for col15a1b, ccnd1, atoh7 and nr2f5 in 60hpf mutant eyes and eyes from mutants treated with BMS493, normalised to β-actin. Values for each gene from DMSO-treated mutant eyes were set to 1 and fold changes in BMS493-treated eyes were plotted relative to this value (±s.e.).

Expression Data

Genes:	atoh7 ccnd1 col15a1b nr2f5
Fish:	gdf6a^u768/u768
Condition:	chemical treatment: BMS-493
Anatomical Term:	ciliary marginal zone
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gdf6a^u768/u768
Condition:	chemical treatment: BMS-493
Observed In:	ciliary marginal zone
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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