FIGURE

Fig. 4

ID

ZDB-FIG-151229-28

Publication

Hanovice et al., 2015 - N-Ethylmaleimide-Sensitive Factor b (nsfb) Is Required for Normal Pigmentation of the Zebrafish Retinal Pigment Epithelium

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Fig. 4

nsfb is a paralog of human NSF and is ubiquitously expressed during early embryo development. (A) Phylogenetic analysis of the relationship between nsf in vertebrates. This analysis was constructed using Geneious Tree Builder based on an alignment of nsf amino acid sequences from human, chimpanzee, mouse, Xenopus, zebrafish, Drosophila, C. elegans, and yeast, using yeast Origin recognition complex subunit 1 (ORC1) as an outgroup. Numbers at nodes represent bootstrap values after 1000 replicates. (B) In situ hybridization reveals that nsfb expression is widespread at 24 hours after fertilization, with higher levels of staining in the somites and nervous system. At 48 hpf, nsfb remains widely expressed in the head, with high levels of expression in the otic vesicle and pectoral fins, but is less apparent in the somites and posterior trunk. Lateral and dorsal views of embryos stained for antisense probe are on top, and sense controls on bottom. Scale bar: 200 µm.

Expression Data

Gene:	nsfb
Fish:	WT
Anatomical Terms:	head nervous system otic vesicle pectoral fin pectoral fin bud somite
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.