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Fig. 4 nsfb is a paralog of human NSF and is ubiquitously expressed during early embryo development. (A) Phylogenetic analysis of the relationship between nsf in vertebrates. This analysis was constructed using Geneious Tree Builder based on an alignment of nsf amino acid sequences from human, chimpanzee, mouse, Xenopus, zebrafish, Drosophila, C. elegans, and yeast, using yeast Origin recognition complex subunit 1 (ORC1) as an outgroup. Numbers at nodes represent bootstrap values after 1000 replicates. (B) In situ hybridization reveals that nsfb expression is widespread at 24 hours after fertilization, with higher levels of staining in the somites and nervous system. At 48 hpf, nsfb remains widely expressed in the head, with high levels of expression in the otic vesicle and pectoral fins, but is less apparent in the somites and posterior trunk. Lateral and dorsal views of embryos stained for antisense probe are on top, and sense controls on bottom. Scale bar: 200 µm.