FIGURE

Fig. 5

ID

ZDB-FIG-151214-30

Publication

Dutta et al., 2015 - nlz1 Is required for cilia formation in zebrafish embryogenesis

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Fig. 5

wnt11 rescues cilia defects innlz1morphant fish (A), and (B) Whole mount in situ hybridization at 3S stage with wnt11 antisense RNA probe as indicated in (A) control MO injected, and (B) Nlz1 MO injected embryos. (C) Bar diagram demonstrating down-regulation of wnt11 expression (~2.5 fold ) in nlz1 morphant embryos compare to control. (D)–(F) Cilia were labeled with anti-acetylated tubulin in KV, and (G)–(I) in FP. Motile cilia were absent in KV and FP of nlz1 morphants (E, n=28/32, H, n=17/24), compared to control MO injected embryos (D, n=40/40, G, n=40/40). Co-injection of wnt11 mRNA and Nlz1 MO (F, n=20/41, I, n=25/41) partially rescues cilia in KV and the FP. (A), and (B) posterior view. KV; Kupffer’s vesicle; s, somite; FP, floor-plate. Scale bar A and B, 100 µm; (D)–(F), 12.5 µm; and (G)–(I), 50 µm.

Expression Data

Gene:	wnt11f2
Fish:	WT WT + MO1-znf703
Knockdown Reagent:	MO1-znf703
Anatomical Terms:	forerunner cell group Kupffer's vesicle whole organism
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-znf703
Knockdown Reagent:	MO1-znf703
Observed In:	determination of heart left/right asymmetry determination of left/right symmetry floor plate motile cilium forerunner cell group Kupffer's vesicle Kupffer's vesicle motile cilium whole organism
Stage Range:	1-4 somites to 5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 406(2), Dutta, S., Sriskanda, S., Boobalan, E., Alur, R.P., Elkahloun, A., Brooks, B.P., nlz1 Is required for cilia formation in zebrafish embryogenesis, 203-11, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.