ZFIN Image: Dutta et al., 2015, Fig. 5

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Figure Caption

Fig. 5

wnt11 rescues cilia defects innlz1morphant fish (A), and (B) Whole mount in situ hybridization at 3S stage with wnt11 antisense RNA probe as indicated in (A) control MO injected, and (B) Nlz1 MO injected embryos. (C) Bar diagram demonstrating down-regulation of wnt11 expression (~2.5 fold ) in nlz1 morphant embryos compare to control. (D)–(F) Cilia were labeled with anti-acetylated tubulin in KV, and (G)–(I) in FP. Motile cilia were absent in KV and FP of nlz1 morphants (E, n=28/32, H, n=17/24), compared to control MO injected embryos (D, n=40/40, G, n=40/40). Co-injection of wnt11 mRNA and Nlz1 MO (F, n=20/41, I, n=25/41) partially rescues cilia in KV and the FP. (A), and (B) posterior view. KV; Kupffer’s vesicle; s, somite; FP, floor-plate. Scale bar A and B, 100 µm; (D)–(F), 12.5 µm; and (G)–(I), 50 µm.

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Acknowledgments

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Reprinted from Developmental Biology, 406(2), Dutta, S., Sriskanda, S., Boobalan, E., Alur, R.P., Elkahloun, A., Brooks, B.P., nlz1 Is required for cilia formation in zebrafish embryogenesis, 203-11, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.