FIGURE

Fig. S1

ID

ZDB-FIG-151208-8

Publication

Teh et al., 2015 - Modulating expression level of secreted Wnt3 influences cerebellum development in zebrafish transgenics

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Functional overexpression of recombinant Wnt3. The recombinant Wnt3 zebrafish fusion protein with EGFP at the C-terminal (Wnt3EGFP) is functional as overexpression in zebrafish produces a posteriorized brain phenotype typical of overt Wnt activity. (A) Bright field image of a malformed embryo with a posteriorized brain upon overt Wnt3EGFP expression under the ubiquitous CMV promoter. Injection of the Wnt3EGFP expression construct at blastula stage resulted in the deformed phenotype. (B) Tg(memKR15-16) expressed membrane localized KillerRed in the midbrain hindbrain boundary (mhb) was used to characterize the deformed brain phenotype. (C) The posteriorized brain phenotype is accompanied by the loss of the midbrain hindbrain boundary observed in Tg(memKR15-16) upon overt Wnt3EGFP expression.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development