FIGURE

Fig. 7

ID

ZDB-FIG-151208-6

Publication

Teh et al., 2015 - Modulating expression level of secreted Wnt3 influences cerebellum development in zebrafish transgenics

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Wnt3EGFP secretion is affected by the block of Porcupine. (A,B) Confocal images of zebrafish cerebellum expressing Wnt3EGFP and LynEGFP after C59 treatment. The embryos were treated with 5µM C59 10-28hpf (for details, see Materials and Methods). The samples were briefly soaked in 1× egg water before imaging in dorsal view, and FCS measurement. Scale bars: 50µm. (C-E) Diffusion coefficients (D₁, D₂) and protein membrane distribution (F₂) extracted from fit at different conditions for both Wnt3EGFP and LynEGFP. (F) The results show that FCS signatures remain unchanged for LynEGFP, indicating that properties of the plasma membrane are not influenced by treatment or the drug function. Data are mean±s.d. Red bar, Wnt3EGFP; green bar, LynEGFP. Significance level, two-way t-test, *P<0.001. See also Table S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development