Fig. 2

Wntless deficiency causes jaw cartilage defects that resemble the defects in wnt5b mutants. (A–D) Lateral views showing the head of 96 hpf WT (A), wls-MO-injected (wls^MO) (B), wnt5b mutant (wnt5b^mt) (C) and wls^MO plus wls mRNA double injected (wls^MO+wls mRNAs) (D) larvae. (E–J) Images showing Alcian-Blue-stained jaw cartilages for (E,F) WT, (G,H) wls^MO, (I,J) and wls^MO plus wls mRNAs larvae at 96 hpf. Arrows indicate the Meckel′s cartilages. (K–N) Images showing wls control MO (5mis-wls^MO) (K,M) or wls MO-injected (L,N) embryos labeled with anti-Wls (red) plus anti-acetylated tubulin (green) antibodies at 24 (K,L) or 48 (M,N) hpf. (O) Cell lysates from control MO (left lane) or wls MO-injected (right lane) embryos detected by anti-Wls antibody in a western blot. (P–S) Images showing 72h (P), 0.5h (Q), 1.25h (R) and 4h (S) embryos labeled by anti-Wls antibodies (red) plus DAPI (blue in Q–S). Images are lateral views in A–D and E,G,I, ventral views in F,H and J, and a dorsal view in P. Numbers in the bottom left corners in E–S indicate the number of the embryos showing the indicated phenotype and the total number examined (denominator).