Fig. 4

Wntless is required for Wnt5b secretion. (A) Cartoon showing cross-section view of the developing head of 48hpf embryos. E, eye; H, hypophysis; M, mesencephalon; P, denotes pharyngeal arch. Dorsal (D) is to the left, ventral (V) to the right. The green box indicates the pharyngeal region shown in images B–E. The red box indicates the region shown in images F–K. (B–E) Confocal images showing sections from the developing jaw of control-MO-injected (5mis-wls^MO) (B,D) wls-MO-injected (wls^MO) (C,E) embryos labeled by anti-β-catenin (in green) plus anti-Wnt5b (red in B,C) or anti-Wnt11 (red in D,E). (F–Q) Images showing enlarged pharyngeal regions from B–E. The arrow in M indicates the regions where Wnt5b is not accumulated on the cell membranes. All images were collected from embryos at 48hpf. Numbers in the bottom left corners in F–Q indicate the number of the embryos showing the indicated phenotype and the total number examined (denominator).