FIGURE

Fig. 8

ID

ZDB-FIG-151029-12

Publication

Mastracci et al., 2015 - Polyamine biosynthesis is critical for growth and differentiation of the pancreas

Other Figures

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Fig. 8

Pharmacological inhibition of DHS with GC7 phenocopies genetic loss of dhps and inhibition of ODC.

(a) Inhibitor treatment strategy. Representative maximum intensity projection images of the pancreas from (b) control and (c) GC7-treated Tg(ptf1a:gfp) embryos; for comparison single channel images are also provided for ptf1a:GFP expression in (b′) control and (c′) GC7-treated embryos, and insulin expression in (b′′) control and (c′′) GC7-treated embryos. (d) Quantification of exocrine pancreas length in control (n = 12) and GC7-treated (n = 15) Tg(ptf1a:gfp) embryos at 72 hpf (p < 0.0001); this represents a 40% difference in exocrine pancreas length. (e) Quantification of insulin + β cell number in control (n = 9) and GC7-treated (n = 13) Tg(ptf1a:gfp) embryos at 72 hpf (p = 0.0556). *p < 0.05, **p < 0.01, ***p < 0.001.

Expression Data

Genes:	EGFP ins
Fish:	jh1Tg
Condition:	chemical treatment by environment: 2-(7-aminoheptyl)guanidine
Anatomical Terms:	endocrine pancreas pancreas
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh1Tg
Condition:	chemical treatment by environment: 2-(7-aminoheptyl)guanidine
Observed In:	endocrine pancreas endocrine pancreas development exocrine pancreas exocrine pancreas development pancreas pancreatic B cell primary islet
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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