FIGURE

Fig. 5

ID

ZDB-FIG-151029-9

Publication

Mastracci et al., 2015 - Polyamine biosynthesis is critical for growth and differentiation of the pancreas

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Fig. 5

Knockdown of dhps in the developing zebrafish.

(a) Design of the dhps splice-blocking morpholino (MO). (b) Injection of the dhps MO results in a loss of exon 2, as confirmed by RT-PCR from control and MO-injected Tg(ptf1a:gfp) embryos; wildtype dhps, 385 bp; E2 deletion in dhps, 172 bp. (c) dhps MO-injected Tg(ptf1a:gfp) embryos at 72 hpf show a significant pancreatic phenotype after low dose MO injection (1 ng or 2 ng). E, exon; bp, base pair.

Expression Data

Gene:	EGFP
Fish:	jh1Tg jh1Tg + MO1-dhps
Knockdown Reagent:	MO1-dhps
Anatomical Terms:	hindbrain pancreas retina
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh1Tg + MO1-dhps
Knockdown Reagent:	MO1-dhps
Observed In:	endocrine pancreas development exocrine pancreas pancreas
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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