FIGURE

Fig. 3

ID

ZDB-FIG-151016-3

Publication

Frese et al., 2015 - RNA splicing regulated by RBFOX1 is essential for cardiac function in zebrafish

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Fig. 3

rbfox1 deficency does not influence heart morphology. (A,B) H&E-stained sagittal histological sections of MO-control and MO1-rbfox1 morphant hearts at 72hpf. Morphants display normal heart morphology with distinct endocardial and myocardial cell layers in the atrium (A) and ventricle (V), and a clear differentiation and demarcation of the atrium and ventricle by the atrio-ventricular ring (AV). OFT, outflow tract. (C,D) Atrial- and ventricle-specific myosin heavy chains are expressed normally, also suggesting that there is normal molecular chamber specification [green, antibody against atrial-specific myosin (S46); red, antibody against ventricular and atrial myosin (MF20)]. (E–H) Ultrastructural analysis of heart and skeletal muscle cells of MO-control- and MO1-rbfox1-injected embryos at 48hpf showing organized sarcomere units (SU) with thin and thick myofilaments in well-aligned bundles and discernible AI-, M- and Z-bands.

Expression Data

Antibodies:	Ab-MF20 Ab-S46
Fish:	WT WT + MO3-rbfox1
Knockdown Reagent:	MO3-rbfox1
Anatomical Terms:	atrium cardiac ventricle
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-rbfox1
Knockdown Reagent:	MO3-rbfox1
Observed In:	atrium cardiac muscle cell heart sarcomere skeletal muscle cell
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.