ZFIN Image: Frese et al., 2015, Fig. 3

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Fig. 3

rbfox1 deficency does not influence heart morphology. (A,B) H&E-stained sagittal histological sections of MO-control and MO1-rbfox1 morphant hearts at 72hpf. Morphants display normal heart morphology with distinct endocardial and myocardial cell layers in the atrium (A) and ventricle (V), and a clear differentiation and demarcation of the atrium and ventricle by the atrio-ventricular ring (AV). OFT, outflow tract. (C,D) Atrial- and ventricle-specific myosin heavy chains are expressed normally, also suggesting that there is normal molecular chamber specification [green, antibody against atrial-specific myosin (S46); red, antibody against ventricular and atrial myosin (MF20)]. (E–H) Ultrastructural analysis of heart and skeletal muscle cells of MO-control- and MO1-rbfox1-injected embryos at 48hpf showing organized sarcomere units (SU) with thin and thick myofilaments in well-aligned bundles and discernible AI-, M- and Z-bands.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.