FIGURE

Fig. 6

ID

ZDB-FIG-151016-4

Publication

Frese et al., 2015 - RNA splicing regulated by RBFOX1 is essential for cardiac function in zebrafish

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Fig. 6

Correctly balanced huG and actn3a isoform expression is essential for proper heart function. (A,B) Lateral view of a MO-huG-injected (MO-zhug) zebrafish embryo at 48hpf. Fractional shortening (FS) shows a progressive reduction of cardiac contractility. Inset, cDNA splice-site analysis of huG morphant. (C,D) Splice-site blockage in actn3a leads to mild cardiac dysfunction. actn3a-depleted zebrafish embryos develop heart failure with dilation of the atrium and reduced ventricular contractility beginning at the 72h developmental stage. Inset, cDNA splice-site analysis of actn3a morphant. Results in B and D are mean±s.d. (n=10).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-actn3a WT + MO1-elavl1b
Knockdown Reagents:	MO1-actn3a MO1-elavl1b
Observed In:	atrium blood circulation cardiac ventricle forebrain heart hindbrain midbrain pericardium
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.