Fig. 6

Mmp9 regulates caudal fin wound healing following amputation. (A) Morpholino knockdown of mmp9 (MO1) causes a regenerative defect at 3dpw without influencing developmental fin length. (B) Co-injection of mmp9 MO2 and zebrafish mmp9 RNA (125ng/µl) rescues the healing defect at 3dpw (5dpf). (C) Schematic of two-site CRISPR-Cas9 targeting of mmp9 (top). Half-stars indicate CRISPR sites and arrows represent primer binding for screening. PCR amplification results in the presence of a ~400bp amplicon indicated by the star, with the ctl lane representing a housekeeping gene to ensure the presence of gDNA (bottom). (D) Mosaic F0 larvae did not have altered fin lengths at 5dpf but displayed a significant defect in caudal fin wound healing. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. Graphs represent a single experiment performed in triplicate. Scale bars: 100µm in A,D.