FIGURE

Fig. 2

ID

ZDB-FIG-150820-2

Publication

LeBert et al., 2015 - Matrix metalloproteinase 9 modulates collagen matrices and wound repair

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Fig. 2

Inflammatory phenotypes associated with hai1 mutants are partially rescued by knockdown of mmp9. (A) Sudan Black staining of the hai1 mutants show increased neutrophilic infiltration of the epithelium (arrows), a phenotype that could be rescued by mmp9 knockdown. (B) Western blot analysis of mmp9 MO1. (C) Morpholino knockdown of mmp9 (MO1) decreased the proportion of hi2217 and hai1 morphants displaying epithelial extrusions and (D) decreased the number of neutrophils infiltrating the epithelium in the hai1 morphants. (E) MMPSense showed hyper-activation of MMPs in the hai1 morphants. Hyper-activation of MMPs in the hai1 morphants could be partially rescued by the knockdown of mmp9 expression (MO1). *P<0.05, **P<0.01 and ****P<0.0001. Scale bars: 300µm in A; 40µm in E. C and D represent data from a single experiment performed in triplicate.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-spint1a WT + MO2-spint1a + MO4-mmp9
Knockdown Reagents:	MO2-spint1a MO4-mmp9
Observed In:	epithelium epithelium neutrophil epithelium development
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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