FIGURE

Fig. 6

ID

ZDB-FIG-150717-18

Publication

Gallardo et al., 2015 - Phenotype-driven chemical screening in zebrafish for collective cell migration inhibitors identifies multiple potential pathways for targeting metastasis

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Fig. 6

Overexpression of src mRNA rescues PLLp migration disrupted by Src signaling pathway inhibitors. Phenotypes of 48hpf cldnb:EGFP embryos upon overexpression of src followed by treatment with Src inhibitors that had been identified in the screen. Overexpression of full-length (fl) src in embryos following DMSO treatment show a normal lateral line development (G). Overexpression of src partially rescued primordium migration defects in embryos treated with RBI (D) and SU6656 (F) compared with non-injected-embryos (C,E), respectively. This phenotype was not rescued in Emodin-treated embryos upon overexpression of src (A,B). (H) Quantification of the average primordium position in drug-treated embryos upon src overexpression relative to the distance from the otic vesicle (ov) to the tip of the tail. Experiments were carried out with ten larvae per condition. Error bars indicate +s.d. ***P<0.001; *P<0.05. mRNA (100pg/2nl) was injected into one-cell stage embryos. n.i., non-injected-embryo.

Expression Data

Gene:	EGFP
Fish:	zf106Tg
Conditions:	chemical treatment: emodin chemical treatment: SU6656
Anatomical Terms:	lateral line neuromast posterior lateral line
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf106Tg
Conditions:	chemical treatment: emodin chemical treatment: SU6656
Observed In:	posterior lateral line development posterior lateral line neuromast deposition
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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