FIGURE

Fig. 4

ID

ZDB-FIG-150717-16

Publication

Gallardo et al., 2015 - Phenotype-driven chemical screening in zebrafish for collective cell migration inhibitors identifies multiple potential pathways for targeting metastasis

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Fig. 4

Blocking the Src signaling pathway inhibits collective cell migration in vivo. Cldnb:EGFP embryos were treated at 36hpf (primordium has migrated beyond the yolk extension, dashed line) with the c-Src inhibitors Emodin (B), RBI (C) or SU6656 (D). Treated embryos show a disruption of the primordium migratory ability compared with DMSO control (A), resulting in a phenotype with a premature deposition of a terminal neuromasts (L6) in all conditions (B-D). (E) Quantification of average position of the last five deposited neuromasts in drug treated-embryos relative to the distance from the ov to the tip of the tail. Arrows indicate the position of the PLLp. Experiments were carried out with ten larvae per condition. Error bars indicate +s.d.; ***P<0.001.

Expression Data

Gene:	EGFP
Fish:	zf106Tg
Conditions:	chemical treatment: emodin chemical treatment: SU6656
Anatomical Terms:	lateral line neuromast
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf106Tg
Conditions:	chemical treatment: emodin chemical treatment: SU6656
Observed In:	posterior lateral line development posterior lateral line neuromast deposition
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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