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Fig. 2 Angptls genetically interact with notch.
(A) Notch eGFP Tg reporter embryos injected with angptls-MO (2 ng) had decreased notch activity in ISV (black arrowheads) and DA (red arrowhead) at 28hpf. (B) Overexpression of constitutively active notch in angptls-MO injected (2 ng) Tg(hsp70:Gal4;UAS:NICD) can restore cmyb-positive HSPCs (arrows) at 36hpf after heatshock. (C) Mib had no cmyb-positive HSPCs (arrows) at 36hpf compared to WT or het siblings. Overexpression of zangptl2 in mib by crossing Tg(hsp70:zangptl2) into mib can restore this defect upon heatshock. Scale bars: 50 µm.
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