FIGURE

Fig. 7

ID

ZDB-FIG-150413-14

Publication

Ma et al., 2015 - Jag1b is essential for patterning inner ear sensory cristae by regulating anterior morphogenetic tissue separation and preventing posterior cell death

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Fig. 7

Inhibition of MAPK signaling generates a larger crista. (A-D) DIC confocal imaging of CI-1040-treated jag1b^+/- embryos at 3dpf. Kinocilia are indicated by arrowheads. AC, anterior crista; LC, lateral crista. (E-H′′) Phalloidin staining of MAPKK inhibitor CI-1040-treated jag1b^+/- embryos at 3dpf. Lateral views. Anterior towards the left and dorsal upwards. Scale bars: 40µm. Arrows and arrowheads indicate cells between AC and LC (E′′-G′′). (I) Whole-mount in situ hybridization of DMSO-and CI-1040-treated embryos. The probes used is msxC antisense. Arrowheads indicate cristae. (J) Total kinocilia number in the anterior and lateral cristae of DMSO-treated embryos and kinocilia number in the fused crista of CI-1040-treated embryos. Data are shown as mean±s.e.m. Statistical analysis was performed using a t-test. n indicates the number of inner ears for each experimental group.

Expression Data

Genes:	EGFP msx3
Fish:	jag1b^gd2Gt/+
Condition:	chemical treatment: pharmaceutical
Anatomical Terms:	otic vesicle anterior crista otic vesicle lateral crista
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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