Fig. 5

A role for rab5ab in Nodal signalling. Lateral view of (A) shield stage control injected embryo compared to (B) shield stage 1.5 ng rab5ab mRNA overexpressing embryo (n=14/41). Lateral view of (C) 24 hpf control injected embryo compared to (D) 24 hpf 1.5 ng rab5ab mRNA overexpressing embryo (n=12/39). Lateral view of (E) 5dpf control injected embryo compared to (F) 5dpf 1.5 ng rab5ab mRNA overexpressing embryo (n=38/38). Expression of gsc in control embryos at (G) 30%, (I) 50%, (K) 70% and (M) 90% epiboly compared to expression of gsc in rab5ab overexpressing embryos at (H) 30% (n=10/12), (J) 50% (n=20/21), (L) 70% (n=8/13) and (N) 90% epiboly (n=5/10). Expression of ntl in control embryos at (O) 30%, (Q) 50%, (S) 70% and (U) 90% epiboly compared to expression of ntl in rab5ab overexpressing embryos at (P) 30% (n=8/12), (R) 50% (n=21/22), (T) 70% (n=9/13) and (V) 90% epiboly (n=7/10). Expression of chd in control embryos at (W) 30%, (Y) 50%, (AA) 70% and (AC) 90% epiboly compared to expression of ntl in rab5ab overexpressing embryos at (X) 30% (n=12/12), (Z) 50% (n=11/11), (AB) 70% (n=10/10) and (AD) 90% epiboly (n=10/10). The gsc expression patterns are shown as animal pole views as are 30% epiboly ntl expressing embryos and 30% and 50% chd expressing embryos. The remainder of the embryos are shown as a side view for improved visualisation of expression patterns.