Fig. 6
- ID
- ZDB-FIG-150317-37
- Publication
- Gutzman et al., 2015 - Non-muscle myosin IIA and IIB differentially regulate cell shape changes during zebrafish brain morphogenesis
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Actin distribution is dependent upon non-muscle myosin II function. (A-F) Embryos were injected as indicated and stained with phalloidin (green) to determine actin localization and regional enrichment. (A-C) Control morphant embryos with normal actin distribution at the MHB. Boxes indicate areas of comparison for determining the mean actin intensity ratio. (A) Comparison of actin intensity with the neuroepithelium at the MHBC region (box 1) compared to the posterior MHB (box 2). (B) Comparison of actin intensity apically at the midline (box 1) compared to the apical region within the neuroepithelial tissue (box 2). (C) Comparison of actin intensity on the basal side of the neuroepithelium (box 1) compared to the basal region within the neuroepithelial tissue (box 2). (G) Quantification of actin distribution comparison ratios for control MO, myh9b MO, myh10 MO, and mypt1 MO injected embryos in the regions indicated. One-way ANOVA with multiple t-test comparisons was performed to determine significance between control and test groups. Results are shown as±s.e.m. Asterisks indicate significance compared to control, p<0.001. Double asterisks indicate, p<0.01. Arrowheads indicate MHBC. Control MO, n=8; myh9b MO, n=12; myh10 MO, n=13; mypt1 MO, n=8. |
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| Stage: | 20-25 somites |
Reprinted from Developmental Biology, 397(1), Gutzman, J.H., Sahu, S.U., Kwas, C., Non-muscle myosin IIA and IIB differentially regulate cell shape changes during zebrafish brain morphogenesis, 103-15, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.