FIGURE

Fig. 4

ID

ZDB-FIG-150317-31

Publication

Gutzman et al., 2015 - Non-muscle myosin IIA and IIB differentially regulate cell shape changes during zebrafish brain morphogenesis

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Fig. 4

myh9b is required for cell shortening at the MHBC during morphogenesis. (A-F) Live confocal images showing the MHB region of 24 ss zebrafish embryos injected with mGFP and coinjected with control MO (A-B), myh9a MO (C), myh9b MO (D), myh10 MO (E), or mypt1 MO (F). (G) Quantification of the cell length (X) at the MHBC (see lines in A, C-F). (H) Quantification of cell length 40 µm outside of the MHBC (Y) (see lines in B-F). One-way ANOVA with multiple t-test comparisons was performed to determine significance between control and test groups. Asterisks indicate p<0.001. Double asterisks indicate p<0.05. Results are shown as±s.e.m. Control MO, n=48; myh9a MO, n=10; myh9b MO, n=16; myh10 MO, n=18; mypt1 MO, n=20. Scale bars: 25 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-myh10 AB + MO2-ppp1r12a AB + MO3-myh9a
Knockdown Reagents:	MO1-myh10 MO2-ppp1r12a MO3-myh9a
Observed In:	midbrain hindbrain boundary cell
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 397(1), Gutzman, J.H., Sahu, S.U., Kwas, C., Non-muscle myosin IIA and IIB differentially regulate cell shape changes during zebrafish brain morphogenesis, 103-15, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.