FIGURE

Fig. 5

ID

ZDB-FIG-150216-6

Publication

Joya et al., 2014 - Transient Exposure to Ethanol during Zebrafish Embryogenesis Results in Defects in Neuronal Differentiation: An Alternative Model System to Study FASD

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Fig. 5

Analysis of cell proliferation and apoptotic cells in control and ethanol-treated embryos.

(A-B) anti-pH3 immunofluorescence at 24hpf in order to analyse cell proliferation. (C-J) Cell death visualization using TUNEL assay in 36hpf embryos (C-D), and 5dpf larvae (E-J). Tg[Mü4127:mCherry] embryos were use for landmarks of r3 and r5 in red. (E,H) Apoptotic activity was visualized in whole-mount embryos at 5dpf. Note that ethanol-treated embryos have more apoptotic figures. (F-I) TUNEL analysis (green) in 5dpf embryos displaying red in the primary sensory neurons (F-F2, I-I2) or in motoneurons (G,J). All are lateral views with anterior to the left.

Expression Data

Genes:	GFP mCherry
Fish:	rw0Tg zc7Tg zf278Et
Condition:	chemical treatment: ethanol
Anatomical Terms:	primary motor neuron rhombomere 3 rhombomere 5 spinal cord sensory neuron
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT zc7Tg
Condition:	chemical treatment: ethanol
Observed In:	apoptotic process sensory neuron apoptotic process
Stage:	Day 5

Phenotype Detail

Acknowledgments

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