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Fig. 5 Analysis of cell proliferation and apoptotic cells in control and ethanol-treated embryos.
(A-B) anti-pH3 immunofluorescence at 24hpf in order to analyse cell proliferation. (C-J) Cell death visualization using TUNEL assay in 36hpf embryos (C-D), and 5dpf larvae (E-J). Tg[Mü4127:mCherry] embryos were use for landmarks of r3 and r5 in red. (E,H) Apoptotic activity was visualized in whole-mount embryos at 5dpf. Note that ethanol-treated embryos have more apoptotic figures. (F-I) TUNEL analysis (green) in 5dpf embryos displaying red in the primary sensory neurons (F-F2, I-I2) or in motoneurons (G,J). All are lateral views with anterior to the left.