Altered expression of myelin-related genes in mct8-/- embryos is recovered by TH analogs.A. The location of putative thyroid response elements (TRE) in the 7, 5, and 2 kb promoter regions of the zebrafish olig2, p0, and mbp genes, respectively. B-F. Whole-mount ISH experiments detected the spatial mRNA expression in 3 dpf embryos (dorsal views) B. olig2 is predominantly expressed in the prethalamus (PTh) and cerebellum (C). C. mbp is expressed primarily in the anterior and posterior lateral line (ALL and PLL, respectively). D. p0 is primarily expressed in a cluster of cells above the otic vesicle (OV), the HB, and spinal cord (SC) in WT-sibling embryos. E. Low expression levels were detected above the otic vesicle in mct8-/- embryos. F. The expression pattern of p0 was recovered in mct8 mRNA-injected mct8-/- embryos. G. Relative mRNA expression of olig2, mbp, and p0 in 3 dpf WT-sibling, mct8-/-, and mct8 mRNA-injected mct8-/- embryos. Values represented as means ±SEM (standard error of the mean). Statistical significance determined by one-way ANOVA followed by a Tukey test (*p<0.05, **p<0.001). H. Relative mRNA expression of p0 in 3 dpf WT-sibling and mct8-/- embryos treated with 0.5 nM T3, 0.5 nM T4, 0.5 nM TETRAC, 5 nM DITPA, and 0.5 nM TRIAC compared to control (Ctrl, treated with 5×10-6 M NaOH) WT-sibling and mct8-/- embryos. Values represented as means ±SEM. Statistical significance determined by two-way ANOVA followed by a Tukey test. Different letters indicate significant difference.
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