FIGURE

Fig. 6

ID

ZDB-FIG-150108-5

Publication

Huang et al., 2013 - Sequential effects of spadetail, one-eyed pinhead and no tail on midline convergence of nephric primordia during zebrafish embryogenesis

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Fig. 6

PGP midline convergence phenotypes in spt^-/-embryos. ((A)-(C)) Whole-mount in situ hybrydization of wt1a and myoD was stained blue and orange, respectively in 12 (A), 24 (B) and 48 hpf (C) spt^-/- embryos. The scale bar indicates 100 µm; ((A)-(C)) are at the same magnification. All images are dorsal view anterior to the top. (D) Quantification of convergence morphogenetic movements in spt^-/- embryos and comparison with WT. The inter distance of PGP was measured at 11 (n=28), 12 (n=32), 15 (n=31), 24 (n=9), 48 (n=15) hpf. The P values of t-test for all 5 stages are smaller than 0.005. The curve fitting (pink) and R-squared value are displayed on the right-hand corner. A turning point (arrow) to divergence movement at 33.5 hpf is generated from an approximation of the stage that has the minimum inter distance of PGP. Error bars represent SD.

Expression Data

Genes:	myod1 wt1a
Fish:	tbx16^b104/b104
Anatomical Terms:	adaxial cell pronephric glomerulus pronephric mesoderm somite
Stage Range:	5-9 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbx16^b104/b104
Observed In:	convergent extension involved in nephron morphogenesis pronephric glomerulus morphogenesis
Stage Range:	5-9 somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 384(2), Huang, C.J., Wilson, V., Pennings, S., MacRae, C.A., and Mullins, J., Sequential effects of spadetail, one-eyed pinhead and no tail on midline convergence of nephric primordia during zebrafish embryogenesis, 290-300, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.