ZFIN Image: Huang et al., 2013, Fig. 6

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Figure Caption

Fig. 6

PGP midline convergence phenotypes in spt^-/-embryos. ((A)-(C)) Whole-mount in situ hybrydization of wt1a and myoD was stained blue and orange, respectively in 12 (A), 24 (B) and 48 hpf (C) spt^-/- embryos. The scale bar indicates 100 µm; ((A)-(C)) are at the same magnification. All images are dorsal view anterior to the top. (D) Quantification of convergence morphogenetic movements in spt^-/- embryos and comparison with WT. The inter distance of PGP was measured at 11 (n=28), 12 (n=32), 15 (n=31), 24 (n=9), 48 (n=15) hpf. The P values of t-test for all 5 stages are smaller than 0.005. The curve fitting (pink) and R-squared value are displayed on the right-hand corner. A turning point (arrow) to divergence movement at 33.5 hpf is generated from an approximation of the stage that has the minimum inter distance of PGP. Error bars represent SD.

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Acknowledgments

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Reprinted from Developmental Biology, 384(2), Huang, C.J., Wilson, V., Pennings, S., MacRae, C.A., and Mullins, J., Sequential effects of spadetail, one-eyed pinhead and no tail on midline convergence of nephric primordia during zebrafish embryogenesis, 290-300, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.