FIGURE

Fig. 6

ID

ZDB-FIG-141105-4

Publication

Ichimura et al., 2013 - Podocalyxin regulates pronephric glomerular development in zebrafish

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Fig. 6

Efficacy for translational blocking of podocalyxin. The expression of Podocalyxin is determined by using immunohistochemistry with a specific antibody. Immunoreactivity for Podocalyxin is remarkably reduced in both the non-cystic type (B, B′) and the cystic type (C, C′) of glomerulus in 2-dpf podxlATGMO morphants in comparison with control (A, A′). In control larva, immunoreactivity for Podocalyxin is recognized at the entire surface of podocyte cell body (arrowheads in A′′). Podocalyxin immunoreactivity surrounding the podocyte cell body is diminished in both the non-cystic and cystic types of glomeruli (arrowheads in B′′, C′′), although a faint signal is found in a linear pattern along the GBM (arrows in B′′, C′′). (A′′–C′′) images are magnification of the regions which are framed by rectangles in the above images. Asterisks indicate the nuclei of podocytes, which are visualized by staining with DAPI. N, notochord. Bar scales, 10 μm in A-C, A′-C′ ; 5 μm in A′′-C′′.

Expression Data

Gene:	podxl
Antibody:	Ab1-podxl
Fish:	AB AB + MO1-podxl
Knockdown Reagent:	MO1-podxl
Anatomical Term:	pronephric podocyte plasma membrane
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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